Prevalence of Bud Blight of Tomato Caused by Groundnut bud necrosis virus in Tamil Nadu
نویسندگان
چکیده
منابع مشابه
Bud necrosis of groundnut (Arachis hypogaea) in India caused by tomato spotted wilt virus*
Chlorotic ringspots or chlorotic leaf specking, terminal bud necrosis, axillary shoot proliferation and severe stunting of groundnut (Arachis hypogaea) were shown to be caused by tomato spotted wilt virus (TSWV). Cowpea (Vigna unguiculata cv. C-152) was found to be a good assay host. TSWV remained infective in buffered sap of groundnut at a dilution of 10-2’5, after storage for 4 h at room temp...
متن کاملImmunodiagnosis of groundnut and watermelon bud necrosis viruses using polyclonal antiserum to recombinant nucleocapsid protein of Groundnut bud necrosis virus.
In vitro gene expression strategy was used for the production of polyclonal antiserum to the nucleocapsid protein (NP) of Groundnut bud necrosis virus (GBNV). The GBNV NP gene from cowpea isolate was cloned into 6x His-tagged UA cloning vector and expressed in Escherichia coli [M15] cells. The fusion protein was detected in insoluble fraction and was purified by using Ni-NTA agarose resin. The ...
متن کاملEffect of Temperature on Symptoms Expression and Viral RNA Accumulation in Groundnut Bud Necrosis Virus Infected Vigna unguiculata
Background: Groundnut bud necrosis virus (GBNV) (Tospovirus genus, Bunyaviridae family) infects the major crops of solanaceae, leguminosae and cucurbitaceae in India. Temperature is an important factor which influences the plant growth and development under diseased conditions.Objective: In the present study, we evaluated the effect of four different temp...
متن کاملAnatomical Study of Primary Bud Necrosis in Vitis vinifera L. cv. Askari in Winter Dormant Bud
Primary bud necrosis in grapevine is a physiological disorder observed in the compound buds. This study was conducted to investigate the anatomical changes in cv. Askari grapevine in winter dormant buds. Bud samples were taken from Askari grapevine at Sisakht vineyards, south-western of Iran. Compound buds were dissected and assessed under a binocular microscope at 10-40x magnification and a di...
متن کاملNSs Encoded by Groundnut Bud Necrosis Virus Is a Bifunctional Enzyme
Groundnut bud necrosis virus (GBNV), a member of genus Tospovirus in the family Bunyaviridae, infects a large number of leguminosae and solanaceae plants in India. With a view to elucidate the function of nonstructural protein, NSs encoded by the small RNA genome (S RNA), the NSs protein of GBNV- tomato (Karnataka) was over-expressed in E. coli and purified by Ni-NTA chromatography. The purifie...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2018
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2018.711.088